NettetHerein, a relatively rapid and efficient method for isolating liver NPCs from a mouse is described. Using this method, liver sinusoidal endothelial cells, Kupffer cells, natural … Nettet28. jul. 2024 · The count varies depending on the mice and the quality of perfusion . Approximately 300,000–500,000 cells can be collected from a liver of C57Bl/6J mice on a regular chow diet, while 1–2 million cells/mouse can be obtained from C57Bl/6J obese mice (>35 g). In addition, flow cytometry analysis shows that 70–80% cells are positive …
Targeting TIM-1 on CD4 T Cells Depresses Macrophage Activation …
Nettet21. apr. 2010 · NKT cell is a population of unconventional T cells that mediate both innate and adaptive T cell responses. Since NKT cells are most abundant in the liver, much … NettetRESEARCH ARTICLE Sample preparation method for isolation of single-cell types from mouse liver for proteomic studies Wei Liu1, Yufang Hou2, Huahai Chen3, Handong … proof involving angles
Isolation of Kupffer Cells and Hepatocytes from a Single Mouse Liver ...
NettetKupffer cells (KCs) play an essential role in the physiological and pathological functions of the liver. Although the isolation methods of KCs have been well-described, most of them are sophisticated and time-consuming. In addition, these methods are mainly used for isolating the KCs of the human an … Nettet25. feb. 2024 · Here, we elaborate a method for effective isolation of both primary hepatocytes and KC from adult liver of the same mouse. This protocol can be used for isolation of liver cells from both wild-type (WT) and genetically-engineered mice. The principle of the method is based on a two-step collagenase perfusion technique in … NettetEXPERIENCE. 2/2012-present Postdoctoral fellow/Research Investigator. Department of Molecular & Integrative Physiology, University of … lacey helfers